Spatial Transcriptome Analysis

This is the research analysis technique that can simultaneously check spatial information and transcriptome information in tissues.

Spatial transcriptome analysis is the latest genetic sequencing technology, which simultaneously assesses the gene expression of cells and the location of the cells. It can directly analyze the gene expression of the tissue section itself without separation into single cells or RNA extraction. By doing so, you can understand the tissue heterogeneity at the same time by obtaining the unique location information of cells in the tissue, as well as the gene expression information. It has the advantage of being applicable to fresh tissue obtained from surgery, FFPE tissue treated with formalin for tissue preservation, in addition to pre-sectioned tissues.

Spatial Gene Expression analysis

As a CSP (Certified Service Provider) for spatial transcriptomics analysis by 10x Genomics, Macrogen is recognized for quick service delivery and a superior level of performance based on its NGS (Next Generation Sequencing) performance, and is ranked first in Korea and fifth in the world. We are also leading the market with diversified spatial transcriptomics analysis services by rapidly introducing new techniques.

Visium CytAssist

Explore and Discover spatial organization of gene expression

Visium : Sample Preparation

FF
(Fresh Frozen)
FFPE
(Formalin Fixed Paraffin Embedded)
Sectioned slide
Sample type OCT (compound) block Paraffin block FF / FFPE
Section Thickness 10 μm 5 μm FF 10µm / FFPE 5µm
RNA Quality RIN ≥ 7 DV200 ≥ 50% FF RIN ≥ 4 / FFPE DV200 ≥ 30%
Species Any organism (poly A tail) Only Human, Mouse Only Human, Mouse
Quality Check Process
  • Visium-FF : Section (Cryotome) - RNA QC & ROI* check - Tissue optimization test
  • Visium-FFPE : Section (Microtome) - RNA QC & ROI check - Tissue adhesion test
  • Visium CytAssist : RNA QC & ROI check - no tissue optimization, tissue adhesion test

*ROI : Region of interest

Nanostring GeoMx® DSP

Discover Where Biology Happens

Platform GeoMx® Digital Spatial Profiler
Sample FFPE Block (Storage period: 3 years / 35.3mm x 14.1 mm x 4-6 µm / Core Needle Biopsy available)
Fresh Frozen Tissue (Store at -80℃, in cutting, within 6 months/ 35.3mm x 14.1 mm x 4-6 µm )
Panel Human Whole Transcriptome Atlas (hWTA)
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